CRISPR interference‐mediated gene regulation in Pseudomonas putida KT 2440

1 Metabolic and regulatory rearrangements underlying glycerol metabolism in 2 Pseudomonas putida KT 2440 3 4 by 5 6

Molecular characterization of Pseudomonas putida KT2440 rpoH gene regulation.

The rpoH gene of Pseudomonas putida KT2440 encoding the heat-shock sigma factor sigma(32) was cloned and sequenced, and the translated gene product was predicted to be a protein of 32.5 kDa. The unambiguous role of the gene as a sigma factor was confirmed because the cloned P. putida gene complemented the growth defect, at 37 and 42 degrees C, of an Escherichia coli rpoH mutant strain. Primer e...

Knock - out of extracytoplasmic function sigma factor ECF - 10 affects stress resistance 1 and biofilm formation in Pseudomonas putida KT 2440

Regulation of valine catabolism in Pseudomonas putida.

The activities of six enzymes which take part in the oxidation of valine by Pseudomonas putida were measured under various conditions of growth. The formation of four of the six enzymes was induced by growth on d- or l-valine: d-amino acid dehydrogenase, branched-chain keto acid dehydrogenase, 3-hydroxyisobutyrate dehydrogenase, and methylmalonate semialdehyde dehydrogenase. Branched-chain amin...

Independent Formaldehyde Dehydrogenase Gene from Pseudomonas putida

A DNA fragment of 485 bp was specifically amplified by PCR with primers based on the N-terminal sequence of the purified formaldehyde dehydrogenase (EC 1.2.1.46) from Pseudomonas putida and on that of a cyanogen bromide-derived peptide. With this product as a probe, a gene coding for formaldehyde dehydrogenase (fdhA) in P. putida chromosomal DNA was cloned in Escherichia coli DH5ot. Sequencing ...